Incorporation of [2-3H]glycerol into rat brain 1,2-diacyl-sn-glycero-3-phosphorylcholine and 1,2-diacyl-sn-glycerol molecular species in vivo
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چکیده
منابع مشابه
arachidonate decreased in diacyl-sn-glycero-3-phosphoinositol, in alkylacyl- and diacyl-sn-glycero-3- phosphocholine and increased in alkenylacyl- and diacyl-sn-glycero-3-phosphoethanolamine. Upon challenge with Ca2+ ionophore A23187, arachidonate decreased in diacyl-sn-glycero-3-phosphoethanolamine, in diacyl- and alkylacyl-sn-glycero-3-phosphocholine and increased in alkenylacyl-sn-glycero-3-phosphoethanolamine
Platelet stimulation by thrombin or Ca2+ ionophore induces mobilization of arachidonate from lipid stores. We have previously shown that, in [14C]arachidonic acid-prelabelled resting platelets, [14C]arachidonate was transferred from diacyl-sn-glycerophosphocholine to ethanolamine and choline-containing ether phospholipids. This transfer reached an equilibrium after 5 h incubation [Colard, Breto...
متن کاملArachidonate cannot be released directly from diacyl-sn-glycero-3-phosphocholine in thrombin-stimulated platelets.
The origin of the arachidonate released from platelets on stimulation with thrombin was investigated by comparing the specific activities of released arachidonate and of arachidonoyl-containing phospholipids using rat platelets prelabelled with arachidonate. Quantification of the released arachidonate was determined in the presence of BW 755 C, a dual cyclo-oxygenase/lipoxygenase inhibitor, whi...
متن کاملInteraction of sn - Glycerol 3 - Phosphorothioate with Escherichia coli IN VITRO AND IN VIVO INCORPORATION INTO
sn-Glycerol 3-phosphorothioate, a bacteriocidal analog of sn-glycerol 3-phosphate in strains of Escherichia coli with a functioning glycerol phosphate transport system, was investigated for its ability to be incorporated into phospholipid under in vitro and i n vivo conditions. A cell-free particulate fraction from E. coli strain 8 catalyzes the transfer of ~n-[~H]glycerol 3-phosphoro[’”S]thio...
متن کاملBiosynthesis of 1-alkyl-sn-glycero-3-phosphate via adenosine triphosphate:1-alkyl-sn-glycerol phosphotransferase.
The pink portion of the rabbit harderian gland was found to be a rich source of ATP: I-alkyl-sn-glycerol phosphotransferase. The enzyme, associated with the microsomal fraction of the gland, exhibits a pH optimum of ‘7.1. ATP and Mg2f are absolute requirements for enzymatic activity; the apparent K,,, for Mg2+ATP is 1.6 mM. Manganese partially substituted for Mgz+, but the measured rate in the ...
متن کاملEnzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat brain and liver.
The formation of product by ethanolamine phosphotransferases (EC 2.7.8.1) and cholinephosphotransferases (EC 2.7.8.2) in microsomal fractions from brains and livers of mature rats is increased several fold by 1,2-diacyl-sn-glycerols. With the addition of 1-alkyl-2-acyl-sn-glycerols, we have found an 11-fold increase with brain microsomes and a 20-fold increase with lvier microsomes in the synth...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1974
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)36831-0